Recombinant DNA technology (chapter 12)

Genetic technology (chapter 12)

I. Introduction

II. RFLP analysis (pp. 316-317, 339-340)

A. Conservation biology example: Diceros bicornis

B. Restriction fragment length polymorphism analysis (Fig. 12.2)

III. PCR and DNA sequencing and owl monkeys

A. PCR (Fig. 12.33)

B. DNA sequencing of specific gene (Fig. 12.35, 12.36, 12.37)

C. Results?

IV. DNA fingerprinting (VNTRs, Southern blotting)

A. A study of EPCs in yellow hammers (Sundberg and Dixon, 1996)

B. VNTRs (Variable number of tandem repeats)

C. Southern blotting

D. Results?

V. Cloning Recombinant DNA technology

A. Example: transgenic livestock that act as drug factories

B. How does one develop a transgenic animal (Valender et al., 1991)

C. Genes can be introduced into organisms in a variety of ways

1. bacterial vectors

2. yeast vectors (YACs)

3. Injecting DNA directly into animal cells (Fig. 12.23)

4. retroviruses with foreign DNA that infect animal cells

5. biolistic transfection of mitochondria and chloroplasts

VI. Mapping and Sequencing the human genome