I. Background

II. Evidence that DNA is the genetic material

A. Transformation (Griffeth, 1928 and Avery et al., 1944)

B. Phage-labeling experiment (Hershey and Chase, 1952)

III. Components of DNA (Fig. 9.8-9.11, Table 9.1, 9.2)

A. Nucleotides: nucleoside + phosphate group

1. Nucleosides

2. Phosphate group

B. Phosphodiester bonds link nucleotides

IV. Structure of DNA

A. Double-stranded helix

B. Antiparellel nature of DNA

C. Contributions to understanding structure of DNA

1. Watson and Crick (Nature, 1953)

2. Franklin and Wilkins (early 1950s, X-ray crystallography)

3. Chargaff (late 1940s-early 1950s, Chargaff's rule)

I. Semi-conservative model of DNA replication

II. Process of replication

A. What is necessary to initiate replication?

B. Elongation

C. Proof-reading

D. Termination (Fig. 9.37)

III. Eukaryotic DNA replication